hold on tight to what is left
Mar. 18th, 2008 01:04 pm![[personal profile]](https://www.dreamwidth.org/img/silk/identity/user.png)
- The Hush Sounds' Goodbye Blues arrived last night. Just in time to bolster my attempt at Bob/Greta as a side pairing. And to make me decide I need to hear and see more of Chris Faller.
- Clumpy uncooperative cells FAIL, okay? I am not amused, because clumpy cells are not appropriate for use in assays designed to compare confluent, contact-inhibited cells to actively growing cells.
- Also, the New-ish Post-doc? Also fails.
We contracted to make antiserum against a specific antigen, and it costs about $2k, even with a discount as a repeat customer and because we're in a non-profit institution.
We get three bleeds. One of the Post-Doc's assigned tasks is to aliquot it when it arrives (one each month) and then characterize it. Some arrived last Monday. I signed for the package, went in and told him. He was busy, and asked me to put in the 'fridge 'til he could get to it. So I did, thinking that an hour or two wouldn't make a difference, because there was a block of ice in the shipping crate. I found the serum in that same crate a week later. When I pointed it out, he said he was busy, and he'd get to it tomorrow.
There isn't big enough or bold enough font to express the WTF moment I had when he said that.
That's a significant investment in time and money, and it should have been processed the day it arrived. I took care of it, because it's a lab reagent. Jesus fuck, if that bleed is lost because he's so slow at experiments and just FORGETS about things. I just. GAH. It's a waste for the entire lab, not just him, and I'm going to be so pissed. And I'm not even the PI here, so it shouldn't be my concern that it's a loss of time and money and reagent.
I need to work in a bubble, seriously.
*deep breaths*
Then he needed gels, but we were out. He kept asking me when I was going to make some. Um, I have stuff planned. He can learn how to pour gels. And really, that should take less than one hour, not an ENTIRE DAY. What the hell? No wonder he works twelve-hour days, it takes him so fucking long to do things.
Also, we make running buffer 1L at a time, and always leave some for the next person to use. He kept asking, "How much I need?"
"Make a liter. Here's the 10X stock solution, here's a 1L cylinder."
"I run five gel. How much I need?"
"Make one liter."
"Don't need liter. How much for five gel?"
"We always make a liter. Common lab courtesy. Make one liter."
"Oh. I make one liter."
Thank you.
*twitch*
At least the new rotation student doesn't have communication issues. Even if she didn't believe me when I told her to let her cells trypsinize for longer than one minute or they'd never come off the plate.
We're going to have a summer undergrad, too.
My patience is so thin right now, OMG.
Can the day be over now, plz?
- Clumpy uncooperative cells FAIL, okay? I am not amused, because clumpy cells are not appropriate for use in assays designed to compare confluent, contact-inhibited cells to actively growing cells.
- Also, the New-ish Post-doc? Also fails.
We contracted to make antiserum against a specific antigen, and it costs about $2k, even with a discount as a repeat customer and because we're in a non-profit institution.
We get three bleeds. One of the Post-Doc's assigned tasks is to aliquot it when it arrives (one each month) and then characterize it. Some arrived last Monday. I signed for the package, went in and told him. He was busy, and asked me to put in the 'fridge 'til he could get to it. So I did, thinking that an hour or two wouldn't make a difference, because there was a block of ice in the shipping crate. I found the serum in that same crate a week later. When I pointed it out, he said he was busy, and he'd get to it tomorrow.
There isn't big enough or bold enough font to express the WTF moment I had when he said that.
That's a significant investment in time and money, and it should have been processed the day it arrived. I took care of it, because it's a lab reagent. Jesus fuck, if that bleed is lost because he's so slow at experiments and just FORGETS about things. I just. GAH. It's a waste for the entire lab, not just him, and I'm going to be so pissed. And I'm not even the PI here, so it shouldn't be my concern that it's a loss of time and money and reagent.
I need to work in a bubble, seriously.
*deep breaths*
Then he needed gels, but we were out. He kept asking me when I was going to make some. Um, I have stuff planned. He can learn how to pour gels. And really, that should take less than one hour, not an ENTIRE DAY. What the hell? No wonder he works twelve-hour days, it takes him so fucking long to do things.
Also, we make running buffer 1L at a time, and always leave some for the next person to use. He kept asking, "How much I need?"
"Make a liter. Here's the 10X stock solution, here's a 1L cylinder."
"I run five gel. How much I need?"
"Make one liter."
"Don't need liter. How much for five gel?"
"We always make a liter. Common lab courtesy. Make one liter."
"Oh. I make one liter."
Thank you.
*twitch*
At least the new rotation student doesn't have communication issues. Even if she didn't believe me when I told her to let her cells trypsinize for longer than one minute or they'd never come off the plate.
We're going to have a summer undergrad, too.
My patience is so thin right now, OMG.
Can the day be over now, plz?
no subject
Date: 2008-03-18 08:01 pm (UTC)I have class tonight. Again. Boo. I miss you!
no subject
Date: 2008-03-18 08:32 pm (UTC)You are a busy, busy woman, and I miss you too.
But I'm gonna see you in, like, ONE MONTH. :D
no subject
Date: 2008-03-18 08:52 pm (UTC)no subject
Date: 2008-03-19 04:25 am (UTC)no subject
Date: 2008-03-19 02:37 pm (UTC)no subject
Date: 2008-03-19 04:42 am (UTC)no subject
Date: 2008-03-19 02:38 pm (UTC)no subject
Date: 2008-03-19 07:31 am (UTC)They sounds like complete and utter idiots. What is the issue with them and where do they keep finding them???
no subject
Date: 2008-03-19 02:41 pm (UTC)Plus, I have to admit that I am not patient when it comes to telling people the same thing over and over again. Especially when it's someone who has enough work experience that none of this should be news.