i'm in love with my own sins
Dec. 12th, 2008 01:16 pmDid Tom Conrad and Jon Walker coordinate for simultaneous glee?
Also? I've been doing these stupid endogenous RhoA activity assays and getting variable results for two months, and I just talked to a new prof, whose entire thesis project hinged on getting this type of assay to work. Her comment? Oh, your bait isn't folded properly. I spent a good chunk of my graduate career trying to figure that out. Then she handed me a protocol and photocopied some of her lab notes.
It would have been nice to know this BEFORE I spent two months using bait made by the standard (shitty) protocol.
Also? I've been doing these stupid endogenous RhoA activity assays and getting variable results for two months, and I just talked to a new prof, whose entire thesis project hinged on getting this type of assay to work. Her comment? Oh, your bait isn't folded properly. I spent a good chunk of my graduate career trying to figure that out. Then she handed me a protocol and photocopied some of her lab notes.
It would have been nice to know this BEFORE I spent two months using bait made by the standard (shitty) protocol.