Entry tags:
i'm in love with my own sins
Did Tom Conrad and Jon Walker coordinate for simultaneous glee?
Also? I've been doing these stupid endogenous RhoA activity assays and getting variable results for two months, and I just talked to a new prof, whose entire thesis project hinged on getting this type of assay to work. Her comment? Oh, your bait isn't folded properly. I spent a good chunk of my graduate career trying to figure that out. Then she handed me a protocol and photocopied some of her lab notes.
It would have been nice to know this BEFORE I spent two months using bait made by the standard (shitty) protocol.
Also? I've been doing these stupid endogenous RhoA activity assays and getting variable results for two months, and I just talked to a new prof, whose entire thesis project hinged on getting this type of assay to work. Her comment? Oh, your bait isn't folded properly. I spent a good chunk of my graduate career trying to figure that out. Then she handed me a protocol and photocopied some of her lab notes.
It would have been nice to know this BEFORE I spent two months using bait made by the standard (shitty) protocol.