I get that it works for slices of tissue in histology, and you get a global picture of staining via epifluorescence. But we're already talking about a height of less than 10um, and I'm trying to determine volume and intensity of the mitotic spindle and centrosome. Flattening the cell will alter the dimensions that I'm trying to measure.
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timeasmymeasure
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Date: 2013-05-29 04:23 pm (UTC)