I've done spins that long in an analytical ultracentrifuge, but never that long in a preparative one. And by its very nature, the sample size for an analytical run is very small, so rotor and spindle problems are extremely rare - you're more likely to have a problem with your cell or the window or detector or something. For this one? Eh, I don't know. I think they are in the Infectious Diseases division, and viral particle isolation sometimes requires long-ish spins, but 18 hours does seem excessive.
They'd already had problems finding a running centrifuge in their dept (I don't even know, right? It's IM, they should have tons of money and equipment as a clinical (thus, billing) dept.) and were desperate, so we let them use our older 'fuge with their rotor. When I left at ~7:30 or so, it had been running for over three hours at speed with no problems, but apparently something happened.
Re: that other thing. Do you feel like had enough of a rapport to contact Agi (Dr. Schonbrunn) and talk to her? Do you think that would be at all helpful? Or do you want to stay in-program?
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Date: 2008-09-08 05:00 pm (UTC)They'd already had problems finding a running centrifuge in their dept (I don't even know, right? It's IM, they should have tons of money and equipment as a clinical (thus, billing) dept.) and were desperate, so we let them use our older 'fuge with their rotor. When I left at ~7:30 or so, it had been running for over three hours at speed with no problems, but apparently something happened.
Re: that other thing. Do you feel like had enough of a rapport to contact Agi (Dr. Schonbrunn) and talk to her? Do you think that would be at all helpful? Or do you want to stay in-program?