asimplechord (![[personal profile]](https://www.dreamwidth.org/img/silk/identity/user.png)
asimplechord) wrote2013-05-28 03:28 pm
asimplechord) wrote2013-05-28 03:28 pm
what, even?
I was trying to explain to Post-Doc that I have to use the confocal microscope to take slices of the cells I'm imaging because they are mitotic and therefore round like beach-balls, where interphase cells are relatively flat, and that various parts of the cellular structure wouldn't be in focus if I simply focused on one plane.
She suggested that I "squish the coverslip to the slide" to flatten it and get everything in one plane. And she was completely sincere about this being good advice.
I was speechless. Seriously. Squish them flat. Because that'll maintain all the 3D structure I'm trying to investigate. Really. How did she earn a Ph.D.? HOW?
She suggested that I "squish the coverslip to the slide" to flatten it and get everything in one plane. And she was completely sincere about this being good advice.
I was speechless. Seriously. Squish them flat. Because that'll maintain all the 3D structure I'm trying to investigate. Really. How did she earn a Ph.D.? HOW?