asimplechord (![[personal profile]](https://www.dreamwidth.org/img/silk/identity/user.png)
asimplechord) wrote2008-04-18 12:49 pm
asimplechord) wrote2008-04-18 12:49 pm
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he makes my brain hurt
I just don't understand.
The Post-Doc Who Looked Good On Paper But Sucks At Laboratory Biochemistry just asked me where we keep secondary antibody labeled with HRP (peroxidase, makes light when substrate + peroxide is added). I showed him (again; I'm pretty sure The Chief has shown him also -- it's the box labeled ANTIBODIES in Sharpie in the freezer, very hard to see, I know). I know he's done westerns since he's been here, but none of them have worked. He said he used the secondary antibodies labeled "AP".
I just. That's the completely wrong secondary. AP = alkaline phosphatase. The enzyme conjugated to the antibody doesn't DO anything when you put it into the HRP substrate solution.
That would explain why none of his westerns have worked.
He is a post-doc. He has as many years experience as I do. How? HOW is he so fucking backwards?
I need more coffee. Or maybe a beer.
The Post-Doc Who Looked Good On Paper But Sucks At Laboratory Biochemistry just asked me where we keep secondary antibody labeled with HRP (peroxidase, makes light when substrate + peroxide is added). I showed him (again; I'm pretty sure The Chief has shown him also -- it's the box labeled ANTIBODIES in Sharpie in the freezer, very hard to see, I know). I know he's done westerns since he's been here, but none of them have worked. He said he used the secondary antibodies labeled "AP".
I just. That's the completely wrong secondary. AP = alkaline phosphatase. The enzyme conjugated to the antibody doesn't DO anything when you put it into the HRP substrate solution.
That would explain why none of his westerns have worked.
He is a post-doc. He has as many years experience as I do. How? HOW is he so fucking backwards?
I need more coffee. Or maybe a beer.