Jan. 18th, 2013

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~ I fell asleep before the Sharapova/V. Williams match. And the Ferrer/Baghdatis match. I swear, I will watch more than just some Andy Murray beat-downs! Except for how NBCSports is airing Yale/Harvard tonight, and probably somewhere I can find a stream of the UND/Minnesota match up (this weekend is the last ever regular-season conference series between these two teams :( ).

+ Saturday: time to make the trip to ATX for music. HELLO, TADDY PORTER, I AM LOOKING AT YOU. I love indoors shows at Stubb's.

+ My brain. Seriously, this bit about jStaal and eStaal in the N&O (They won’t sit side-by-side in the Canes’ newly renovated locker room at PNC Arena – "He doesn’t want me that close to him all the time," Jordan jokes – but they will see enough of each other.) sent me straight to wondering if Skinner still has the stall next to eStaal, and it all devolved into fic-related ideas from there.

? Science query. I'm doing some RT-PCR, and we spec all our samples with a nanospec before calculating how much to use in each subsequent step of the process. But my question is: if I spec RNA, then do first-strand synthesis for cDNA and spec it again... how does the nanospec differentiate between free, unused dNTPs and ssDNA, which becomes the template for the next step? It's all based on absorbance at 260 and 280, right?

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